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BA-01X – Bridge Amplifier

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Intracellular Bridge Mode Amplifier

  • Save money with this simpler version of the BA-03X
  • Same precise current clamp performance
  • Easy to use cell penetration mode with sharp microelectrodes
  • Compatible with any data acquisition system
  • Also available as module for the EPMS-07 system
  • For additional voltage clamp capability choose the ELC-01X
  • Combine with our miniature headstages for in vivo recording

About BA

The BA amplifiers are wide-bandwidth high impedance amplifier designed for intracellular current clamp recordings with sharp microelectrodes, in perforated or tight seal patch configurations as well as for electroporation and iontophoresis. Currently, npi BA amplifiers are used in vertebrate (rat, mouse, cat, fish etc.) and invertebrate (leech, crab, snail, insect etc.) neurons, muscle cells, other excitable tissue and plant cells.

Each amplifier of the BA series is housed in a 19″ rackmount cabinet and equipped with functions like two ranges for bridge balance, an oscillation shut-off circuit for protecting the preparation, two potential and two current ranges. The amplifier comes with a small headstage with a holding bar (or a mounting plate / dovetail adapter on request) and a BNC connector for the electrode holder, and can be attached directly to a micromanipulator.

The BA-01X is the standard bridge amplifier fot intracellular current clamp recordings. Having an extended current range (x10, i.e. +/. 120 nA max.) this amplifer is also suitable for electroporation of single cells for non-invasive, juxtacellular filling of cells with dyes or plasmids.
The BA-03X has additionally high and low pass filters for the recorded potenial, and a potential gain up to 1000. Therefore, it allows extracellular recordings as well.

A bridge amplifier is also available as module for the EPMS-07 system.

Features

  • True current clamp operation with direct measurement of membrane potential and complete cancellation of series resistance and stray capacitance
  • Can be used with sharp microelectrodes, or patch pipettes in whole-cell, cell-attached and perforated-patch configuration
  • Digital DISPLAYS for current, voltage and electrode resistance
  • BUZZ and ELECTRODE CLEAR facility, AUDIO monitor
  • OSCILLATION SHUT-OFF uni prevents cells from damage
  • TTL gates stimulus input with ampliture setting by 3-digit potentiometer and digital read-out of holding current
  • Enhanced (x10) current range for Juxtracellular recordings, electroporation or Iontophoresis

Ordering

Part No. Description
BA-01X BA-01X 19″ Bridge Mode Amplifier
BA-MODA Cell Model /w Active Membrane Resistance for BA System
BA-MOD Cell Model Circuit (passive) for BA System
BA-EH Standard Micro-/Patch Electrode Holder
BA-EH-SET Electrode Holder Set for BRAMP-System
BA-PRS Remote Control f. PENETRATION Unit
BA-PRS-F Remote Control f. PENETRATION Unit (foot)
BA-HS Headstage for BA System
BA-MINI Miniature headstage for BA-series amplifier. Many different configurations available for in-vivo or in-vitro use, housing and cable options.

Please contact us for further information and prices.

 

Technical Data

HEADSTAGE Input voltage range: ±1000 mV
Operating voltage: ±15 V
Enclosure: Size: 23 x 70 x 26 mm, grounded
Holding bar: Size: length 150 mm, diameter 8 mm
Electrode connector: BNC connector with driven shield
Ground connector: 2.4 mm connector
Input resistance: >1013 Ω
Current range x1: ±12 nA into 100 MΩ
Current range x10: ±120 nA into 100 MΩ
ELECTRODE PARAMETER CONTROLS BIAS: ±150 pA in range x1 and x10, adjustable with ten-turn control
OFFSET: ten-turn control, range ±200 mV
CAPACITY COMPENSATION: range 0-30 pF, ten-turn control.
CELL PENETRATION Overcompensation of capacity compensation (BUZZ), duration ~2 – ~50 ms (slightly dependent on the setting of capacitance compensation), timer controlled with linear control,
accessible with remote switch
ELECTRODE CLEAR CIRCUIT Application of max. DC currents (-Imax or +Imax),
OSCILLATION SHUT-OFF Turns off current injection and capacity compensation, function indicated by red / green LED,
disabled / off / reset switch, threshold set with linear control (0-1200 mV).
BRIDGE BALANCE adjustable with ten-turn control, 0-100 MΩ and 0-1000 MΩ with toggle switch.
ELECTRODE RESISTANCE test 10 mV / MΩ obtained by application of square current pulses ±1 nA, display XXXX MΩ.
BANDWIDTH and SPEED of RESPONSE Full power bandwidth (Re = 0): >30 kHz, rise time (10 % – 90 %) <10 µs (Re = 100 MΩ),
<5 µs (Re = 5 MΩ).
OUTPUTS (BA-01X) Impedance: 50 Ω;
Current x1: sensitivity 0.1 V / nA, display XX.XX nA, resolution 10 pA;
Current x10: sensitivity 0.1V / nA, display XXX.X nA, resolution 100 pA;
Potential: BNC connector, sensitivity x10 mV or x100 mV, selectable with toggle switch, display XXXX mV.
OUTPUTS (BA-03X) Impedance: 50 Ω;
Current range x1: sensitivity 1 V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XX.XX nA, resolution 10 pA;
Current range x10: sensitivity 0.1V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XXX.X nA, resolution 100 pA;
Potential: BNC connector, sensitivity x10 mV to x1000 mV, selectable with rotary switch (7 steps), display XXXX mV.
CURRENT INPUTS RANGE x1 0.1 nA / V, 1 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 10 pA, polarity selectable with toggle switch,
holding current range ±10 nA with ten-turn control.
CURRENT INPUTS RANGE x10 1 nA / V, 10 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 100 pA, polarity selectable with toggle switch,
holding current range ±100 nA with ten-turn control.
POWER REQUIREMENTS 115 / 230 V AC, 25 W
DIMENSIONS 19″ rackmount cabinet, 19″ (483 mm) wide, 10″ (250 mm) deep, 3.5″ (88 mm) high

 

Downloads

Manuals BA-01X_manual
Brochures BA-Series
Juxtacellular Filling

About BA

About BA

The BA amplifiers are wide-bandwidth high impedance amplifier designed for intracellular current clamp recordings with sharp microelectrodes, in perforated or tight seal patch configurations as well as for electroporation and iontophoresis. Currently, npi BA amplifiers are used in vertebrate (rat, mouse, cat, fish etc.) and invertebrate (leech, crab, snail, insect etc.) neurons, muscle cells, other excitable tissue and plant cells.

Each amplifier of the BA series is housed in a 19″ rackmount cabinet and equipped with functions like two ranges for bridge balance, an oscillation shut-off circuit for protecting the preparation, two potential and two current ranges. The amplifier comes with a small headstage with a holding bar (or a mounting plate / dovetail adapter on request) and a BNC connector for the electrode holder, and can be attached directly to a micromanipulator.

The BA-01X is the standard bridge amplifier fot intracellular current clamp recordings. Having an extended current range (x10, i.e. +/. 120 nA max.) this amplifer is also suitable for electroporation of single cells for non-invasive, juxtacellular filling of cells with dyes or plasmids.
The BA-03X has additionally high and low pass filters for the recorded potenial, and a potential gain up to 1000. Therefore, it allows extracellular recordings as well.

A bridge amplifier is also available as module for the EPMS-07 system.

Features

Features

  • True current clamp operation with direct measurement of membrane potential and complete cancellation of series resistance and stray capacitance
  • Can be used with sharp microelectrodes, or patch pipettes in whole-cell, cell-attached and perforated-patch configuration
  • Digital DISPLAYS for current, voltage and electrode resistance
  • BUZZ and ELECTRODE CLEAR facility, AUDIO monitor
  • OSCILLATION SHUT-OFF uni prevents cells from damage
  • TTL gates stimulus input with ampliture setting by 3-digit potentiometer and digital read-out of holding current
  • Enhanced (x10) current range for Juxtracellular recordings, electroporation or Iontophoresis

Ordering

Ordering

Part No. Description
BA-01X BA-01X 19″ Bridge Mode Amplifier
BA-MODA Cell Model /w Active Membrane Resistance for BA System
BA-MOD Cell Model Circuit (passive) for BA System
BA-EH Standard Micro-/Patch Electrode Holder
BA-EH-SET Electrode Holder Set for BRAMP-System
BA-PRS Remote Control f. PENETRATION Unit
BA-PRS-F Remote Control f. PENETRATION Unit (foot)
BA-HS Headstage for BA System
BA-MINI Miniature headstage for BA-series amplifier. Many different configurations available for in-vivo or in-vitro use, housing and cable options.

Please contact us for further information and prices.

 

Technical Data

Technical Data

HEADSTAGE Input voltage range: ±1000 mV
Operating voltage: ±15 V
Enclosure: Size: 23 x 70 x 26 mm, grounded
Holding bar: Size: length 150 mm, diameter 8 mm
Electrode connector: BNC connector with driven shield
Ground connector: 2.4 mm connector
Input resistance: >1013 Ω
Current range x1: ±12 nA into 100 MΩ
Current range x10: ±120 nA into 100 MΩ
ELECTRODE PARAMETER CONTROLS BIAS: ±150 pA in range x1 and x10, adjustable with ten-turn control
OFFSET: ten-turn control, range ±200 mV
CAPACITY COMPENSATION: range 0-30 pF, ten-turn control.
CELL PENETRATION Overcompensation of capacity compensation (BUZZ), duration ~2 – ~50 ms (slightly dependent on the setting of capacitance compensation), timer controlled with linear control,
accessible with remote switch
ELECTRODE CLEAR CIRCUIT Application of max. DC currents (-Imax or +Imax),
OSCILLATION SHUT-OFF Turns off current injection and capacity compensation, function indicated by red / green LED,
disabled / off / reset switch, threshold set with linear control (0-1200 mV).
BRIDGE BALANCE adjustable with ten-turn control, 0-100 MΩ and 0-1000 MΩ with toggle switch.
ELECTRODE RESISTANCE test 10 mV / MΩ obtained by application of square current pulses ±1 nA, display XXXX MΩ.
BANDWIDTH and SPEED of RESPONSE Full power bandwidth (Re = 0): >30 kHz, rise time (10 % – 90 %) <10 µs (Re = 100 MΩ),
<5 µs (Re = 5 MΩ).
OUTPUTS (BA-01X) Impedance: 50 Ω;
Current x1: sensitivity 0.1 V / nA, display XX.XX nA, resolution 10 pA;
Current x10: sensitivity 0.1V / nA, display XXX.X nA, resolution 100 pA;
Potential: BNC connector, sensitivity x10 mV or x100 mV, selectable with toggle switch, display XXXX mV.
OUTPUTS (BA-03X) Impedance: 50 Ω;
Current range x1: sensitivity 1 V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XX.XX nA, resolution 10 pA;
Current range x10: sensitivity 0.1V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XXX.X nA, resolution 100 pA;
Potential: BNC connector, sensitivity x10 mV to x1000 mV, selectable with rotary switch (7 steps), display XXXX mV.
CURRENT INPUTS RANGE x1 0.1 nA / V, 1 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 10 pA, polarity selectable with toggle switch,
holding current range ±10 nA with ten-turn control.
CURRENT INPUTS RANGE x10 1 nA / V, 10 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 100 pA, polarity selectable with toggle switch,
holding current range ±100 nA with ten-turn control.
POWER REQUIREMENTS 115 / 230 V AC, 25 W
DIMENSIONS 19″ rackmount cabinet, 19″ (483 mm) wide, 10″ (250 mm) deep, 3.5″ (88 mm) high

 

Downloads

Downloads

Manuals BA-01X_manual
Brochures BA-Series
Juxtacellular Filling