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BA-01X – Bridge Amplifier

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Intracellular Bridge Mode Amplifier

  • Save money with this simpler version of the BA-03X
  • Same precise current clamp performance
  • Easy to use cell penetration mode with sharp microelectrodes
  • Compatible with any data acquisition system
  • Also available as module for the EPMS-07 system
  • For additional voltage clamp capability choose the ELC-01X
  • Combine with our miniature headstages for in vivo recording
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Features

  • True current clamp operation with direct measurement of membrane potential and complete cancellation of series resistance and stray capacitance
  • Can be used with sharp microelectrodes, or patch pipettes in whole-cell, cell-attached and perforated-patch configuration
  • Digital DISPLAYS for current, voltage and electrode resistance
  • BUZZ and ELECTRODE CLEAR facility, AUDIO monitor
  • OSCILLATION SHUT-OFF uni prevents cells from damage
  • TTL gates stimulus input with ampliture setting by 3-digit potentiometer and digital read-out of holding current
  • Enhanced (x10) current range for Juxtracellular recordings, electroporation or Iontophoresis

Ordering

Part No. Description
BA-01X BA-01X 19″ Bridge Mode Amplifier
BA-MODA Cell Model /w Active Membrane Resistance for BA System
BA-MOD Cell Model Circuit (passive) for BA System
BA-EH Standard Micro-/Patch Electrode Holder
BA-EH-SET Electrode Holder Set for BRAMP-System
BA-PRS Remote Control f. PENETRATION Unit
BA-PRS-F Remote Control f. PENETRATION Unit (foot)
BA-HS Headstage for BA System
BA-MINI Miniature headstage for BA-series amplifier. Many different configurations available for in-vivo or in-vitro use, housing and cable options.

Please contact us for further information and prices.

 

Technical Data

Headstage

Input voltage range: ±1 V
Operating voltage: ±15 V
Enclosure: Size: 23 x 70 x 26 mm, grounded
Dovetail: Size: 70 mm x 17 mm x 3 mm
Electrode connector: BNC with driven shield
REF connector: SMB connector
Ground connector: 2.4 mm connector
Input resistance (CC): >1013 Ω (internally adjustable)
Current range: ±10 nA max. (100 MΩ feedback, X1 CURRENT RANGE)
±100 nA max. (100 MΩ feedback, X10 CURRENT RANGE)

Electrode parameter controls

OFFSET: range ±200 mV, ten-turn control
CAPACITY COMPENSATION: range 0 – 30 pF, ten-turn control
BIAS: range ±150 pA, ten-turn control
BRIDGE BALANCE: 0-100 MΩ (range switch set to 10 MΩ)
0-1000 MΩ (range switch set to 100 MΩ)
adjustable with ten-turn control
Electrode clear circuit: Application of max. DC currents (-Imax or +Imax)
Cell penetration: BUZZ, duration ~2.5 – ~100 ms

Electrode resistance test

Sensitivity 1 mV / MΩ application of square current pulses ±1 nA
Display: 3 ½ digit, XXX MΩ, activated by key switch
(same as POTENTIAL display)

Bandwidth and speed response (CC mode, optimal capacity compensation)

Full power bandwidth (REL = 0 MΩ): >30 kHz
rise time (10% – 90%) <10 µs (REL = 100 MΩ)
<5 µs (REL = 5 MΩ)

Outputs

Output impedance: 50 Ω
Max. voltage: ±12 V
Current output: BNC connector, sensitivity 0.1 V/nA,
Current display: 3 ½ digits, XX.XX nA, resolution 10 pA (X1 CURRENT RANGE)
3 ½ digits, XXX.X nA, resolution 100 pA (X10 CURRENT RANGE)
POTENTIAL OUTPUT FROM HEADSTAGE: BNC connector, sensitivity 1 V/V
POTENTIAL OUTPUT: BNC connector, sensitivity 10 V/V or 100 V/V
Potential output gain: Toggle switch, x10, x100
Potential output resolution in AC: 50 µV

Digital displays:

Display mV/MΩ 3 ½ digits, XXXX mV or XXX MΩ
Display current 3 ½ digits, XX.XX nA (X1 CURRENT RANGE)
3 ½ digits, XXX.X nA (X10 CURRENT RANGE)

Inputs

Input impedance analog 100 kΩ
Input range ±12 V
Input impedance digital (TTL) 10 kΩ
Input range TTL 0-5 V
Current stimulus input CC via BNC connectors
X1 CURRENT RANGE: 1 nA/V or 0.1 nA/V
X10 CURRENT RANGE: 10 nA/V or 1 nA/V
Holding current: X1 CURRENT RANGE: ±10 nA
X10 CURRENT RANGE: ±100 nA
Adjustable with ten-turn control
Step gate input via BNC connector (TTL)
Gated stimulus CC X1 CURRENT RANGE: resolution: 10 pA, range: ±10 nA
X10 CURRENT RANGE: resolution: 100 pA, range: ±100 nA
with digital control of current step size
Polarity selectable with toggle switch

Physical specifications

Dimensions: 19” rackmount cabinet
19” (483 mm), 10” (250 mm), 3.5” (88 mm)
Power requirements: 115/230 V AC, 60/50 Hz, fuse 0.4/0.2 A, slow, 25 W
Weight: 4.0 kg

Downloads

Manuals BA-01X_manual
Brochures BA-Series
Juxtacellular Filling

ABOUT

The BA amplifiers are wide-bandwidth high impedance amplifier designed for intracellular current clamp recordings with sharp microelectrodes, in perforated or tight seal patch configurations as well as for electroporation and iontophoresis. Currently, npi BA amplifiers are used in vertebrate (rat, mouse, cat, fish etc.) and invertebrate (leech, crab, snail, insect etc.) neurons, muscle cells, other excitable tissue and plant cells.

Each amplifier of the BA series is housed in a 19″ rackmount cabinet and equipped with functions like two ranges for bridge balance, an oscillation shut-off circuit for protecting the preparation, two potential and two current ranges. The amplifier comes with a small headstage with a holding bar (or a mounting plate / dovetail adapter on request) and a BNC connector for the electrode holder, and can be attached directly to a micromanipulator.

The BA-01X is the standard bridge amplifier fot intracellular current clamp recordings. Having an extended current range (x10, i.e. +/. 120 nA max.) this amplifer is also suitable for electroporation of single cells for non-invasive, juxtacellular filling of cells with dyes or plasmids.
The BA-03X has additionally high and low pass filters for the recorded potenial, and a potential gain up to 1000. Therefore, it allows extracellular recordings as well.

A bridge amplifier is also available as module for the EPMS-07 system (BA-01M).

Features

Features

  • True current clamp operation with direct measurement of membrane potential and complete cancellation of series resistance and stray capacitance
  • Can be used with sharp microelectrodes, or patch pipettes in whole-cell, cell-attached and perforated-patch configuration
  • Digital DISPLAYS for current, voltage and electrode resistance
  • BUZZ and ELECTRODE CLEAR facility, AUDIO monitor
  • OSCILLATION SHUT-OFF uni prevents cells from damage
  • TTL gates stimulus input with ampliture setting by 3-digit potentiometer and digital read-out of holding current
  • Enhanced (x10) current range for Juxtracellular recordings, electroporation or Iontophoresis

Ordering

Ordering

Part No. Description
BA-01X BA-01X 19″ Bridge Mode Amplifier
BA-MODA Cell Model /w Active Membrane Resistance for BA System
BA-MOD Cell Model Circuit (passive) for BA System
BA-EH Standard Micro-/Patch Electrode Holder
BA-EH-SET Electrode Holder Set for BRAMP-System
BA-PRS Remote Control f. PENETRATION Unit
BA-PRS-F Remote Control f. PENETRATION Unit (foot)
BA-HS Headstage for BA System
BA-MINI Miniature headstage for BA-series amplifier. Many different configurations available for in-vivo or in-vitro use, housing and cable options.

Please contact us for further information and prices.

 

Technical Data

Technical Data

Headstage

Input voltage range: ±1 V
Operating voltage: ±15 V
Enclosure: Size: 23 x 70 x 26 mm, grounded
Dovetail: Size: 70 mm x 17 mm x 3 mm
Electrode connector: BNC with driven shield
REF connector: SMB connector
Ground connector: 2.4 mm connector
Input resistance (CC): >1013 Ω (internally adjustable)
Current range: ±10 nA max. (100 MΩ feedback, X1 CURRENT RANGE)
±100 nA max. (100 MΩ feedback, X10 CURRENT RANGE)

Electrode parameter controls

OFFSET: range ±200 mV, ten-turn control
CAPACITY COMPENSATION: range 0 – 30 pF, ten-turn control
BIAS: range ±150 pA, ten-turn control
BRIDGE BALANCE: 0-100 MΩ (range switch set to 10 MΩ)
0-1000 MΩ (range switch set to 100 MΩ)
adjustable with ten-turn control
Electrode clear circuit: Application of max. DC currents (-Imax or +Imax)
Cell penetration: BUZZ, duration ~2.5 – ~100 ms

Electrode resistance test

Sensitivity 1 mV / MΩ application of square current pulses ±1 nA
Display: 3 ½ digit, XXX MΩ, activated by key switch
(same as POTENTIAL display)

Bandwidth and speed response (CC mode, optimal capacity compensation)

Full power bandwidth (REL = 0 MΩ): >30 kHz
rise time (10% – 90%) <10 µs (REL = 100 MΩ)
<5 µs (REL = 5 MΩ)

Outputs

Output impedance: 50 Ω
Max. voltage: ±12 V
Current output: BNC connector, sensitivity 0.1 V/nA,
Current display: 3 ½ digits, XX.XX nA, resolution 10 pA (X1 CURRENT RANGE)
3 ½ digits, XXX.X nA, resolution 100 pA (X10 CURRENT RANGE)
POTENTIAL OUTPUT FROM HEADSTAGE: BNC connector, sensitivity 1 V/V
POTENTIAL OUTPUT: BNC connector, sensitivity 10 V/V or 100 V/V
Potential output gain: Toggle switch, x10, x100
Potential output resolution in AC: 50 µV

Digital displays:

Display mV/MΩ 3 ½ digits, XXXX mV or XXX MΩ
Display current 3 ½ digits, XX.XX nA (X1 CURRENT RANGE)
3 ½ digits, XXX.X nA (X10 CURRENT RANGE)

Inputs

Input impedance analog 100 kΩ
Input range ±12 V
Input impedance digital (TTL) 10 kΩ
Input range TTL 0-5 V
Current stimulus input CC via BNC connectors
X1 CURRENT RANGE: 1 nA/V or 0.1 nA/V
X10 CURRENT RANGE: 10 nA/V or 1 nA/V
Holding current: X1 CURRENT RANGE: ±10 nA
X10 CURRENT RANGE: ±100 nA
Adjustable with ten-turn control
Step gate input via BNC connector (TTL)
Gated stimulus CC X1 CURRENT RANGE: resolution: 10 pA, range: ±10 nA
X10 CURRENT RANGE: resolution: 100 pA, range: ±100 nA
with digital control of current step size
Polarity selectable with toggle switch

Physical specifications

Dimensions: 19” rackmount cabinet
19” (483 mm), 10” (250 mm), 3.5” (88 mm)
Power requirements: 115/230 V AC, 60/50 Hz, fuse 0.4/0.2 A, slow, 25 W
Weight: 4.0 kg

Downloads

Downloads

Manuals BA-01X_manual
Brochures BA-Series
Juxtacellular Filling

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