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OctaFlow II – Automated Perfusion System

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OctaFlow II – Multi-function Perfusion System

  • Rapid flush mode for repeatable results
  • Programmable solution delivery
  • Keyboard control for fast reaction

About OctaFlowII

Overview

The OctaFlow II ™ from ALA is our most advanced drug application device for electrophysiology and imaging research. This fast perfusion system is designed to meet the needs of basic science and drug discovery researchers. Software control of all perfusion parameters makes the OctaFlow II ™ superior to all other perfusion systems.

Key features of the OctaFlow II ™ include:

    • Choice of low-maintenance pinch valves or fast-response solenoid valves
    • Valve-control of solution flow instead of motorized manipulation of barreled pipettes, minimizing sample losses following solution exchange
    • Keyboard solution selection, for exchange “on the fly”
    • Trigger in /out for synchronization with data acquisition systems
    • Preprogrammed solution delivery through software
    • Rapid flush mode for easy cleaning
    • Programmable pressurization of fluids for optimal solution flow
    • Programmable analog outputs for valve identification, step number, or pressure information to data acquisition

Each OctaFlow II ™ system allows for a choice of 8, 16, 24 or 32 reservoirs, which can be configured in different modes, depending on the experiment(s). Constant perfusion can be achieved through the combination of compound application and wash solution for minimization of solution convergence in manifold. Periodic application of compounds can be achieved through specific focal application. U-tube and J-tube configurations are also possible with the Octaflow II™.

The OctaFlow II ™ software runs on Windows 7 (32-bit) or Windows XP and communicates with the VC3 electronic valve controller via USB 2.0. Sequence steps are loaded at USB 2.0 speeds for fast editing and executing of sequence files. The graphical interface is designed to control all perfusion parameters from a single window. It also allows you to create configuration files and sequence files and to save them for future use. The Cleaning Manager function in the software allows for fast cleaning of reservoirs, valves and tubing.

Details

Each OctaFlow II ™system includes the following:

  • Magnetic stand(s) with poles
  • Valve manifold(s) with solenoid or pinch valves and 5mL pressurized syringe reservoirs
  • Flush valve(s)
  • Quartz MicroManifold® perfusion outlet manifold(s) in various configurations (see below)
  • 230um ID and 600um OD Teflon tubing (FEP-1) and compression fittings (CF-1) for 1/16″ ID tubing (other sizes available – see below)
  • USB cable and software

Each OctaFlow II ™ system features a choice of valves:

  • Pinch valves : The 3-way normally open and normally closed pinch valves require minimal maintenance in long-term perfusion experiments and have response times of 15-20 ms.
  • Solenoid valves : The 2-way normally closed solenoid valves are optimized for high-speed solution exchange. These valves feature a biologically inert internally wettable surface and have response times of 1-2 ms.

Additional options include:

  • CF-2: Compression fitting for PE-20/FEP-2 tubing to 1/16″ ID tubing
  • FEP-2: Clear Teflon tubing with 380um ID and 760um OD
  • QMM-8: Quartz MicroManifold® 8 to 1 channel perfusion outlet manifold with 100um or 200um ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-16: Quartz MicroManifold® 16 to 1 channel perfusion outlet manifold with 100um or 200um ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-24: Quartz MicroManifold® 24 to 1 channel perfusion outlet manifold with 100um or 200um ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-32: Quartz MicroManifold® 32 to 1 channel perfusion outlet manifold with 100um or 200um ID polyimide-coated quartz tubing and tip (other sizes available)

All of the Quartz MicroManifold® perfusion outlet manifolds are also available with an additional channel with 200um ID tube for flushing dead space and/or with Teflon tubing installed.

Ordering

Part No. Description
ALA SPK-8 8 Channel Simple Perfusion Kit: magnetic stand with poles, reservoir bracket, drip chambers, 8 channel perfusion outlet manifold, manual 3-way luer valves, manual pinch flow valves, tubing and accessories
ALA 60MLRESCLP Set of four 60mL reservoir mounting clips with screws
ALA RESBRACK-8 8 Reservoir bracket for 60mL reservoirs with mounting block – other sizes available
ALA MMF-8 MiniManifold TM 8 to 1 channel perfusion outlet manifold made of 1/16″ ID ABS plastic
ALA 60mLSYRINGE Box of 25 60mL syringes
ALA SPK-DRIP Replacement drip chambers – pack of 10
ALA 3WLV Manual 3-way stop cock luer valve
ALA FCV-1 Manual pinch flow valve
ALA VM-STAND Replacement valve manifold stand: magnetic base and 2 rods
ALA SFK Miscellaneous tube and luer fittings
ALA TUBING-2 10′ 1/16″ ID x 1/8″ OD PVC tubing

 

Technical Data

Maximum number of perfusion valves 32
Suction developed via internal Venturi pump 199 mmHg nominal
Maximum number of valves per bank 8 valves/bank, up to 4 banks
Typical speed of pressure rise 520mmHg/sec
Maximum current output per valve 100mA/ 350mA solenoid/pinch valve
Pressures selectable in mm/Hg in increments of 1 or other units selectable
Maximum voltage/valve 18V
Sample flow rate 1mL in 9 min. @ 520 mmHg with standard QMM
Maximum on time per valve no limit
Minimum on time per valve 7ms @ 12V & 1ms @ 18V
Standard QMM 8 tubes with 100μm ID, 1 tube with 200μm ID, 100μm ID tip
Maximum input pressure 3970mmHg – other units selectable
Tip sizes available 100, 150, 200 μm ID
Maximum pressure applied ~800mmHg
Maximum number of sequence steps 300
Power requirements 120/220V
Maximum number of sequence events with looping 30020
OctaFlow interface 19″x3.5″x10″, 10 lbs/4.5kg
Infinite Step Function in Repeat Mode
Programmable Voltage Range 1-22volts/valve
USB 2.0 Support Software is compatible with Windows 7 and Windows XP

About OctaFlowII

About OctaFlowII

Overview

The OctaFlow II ™ from ALA is our most advanced drug application device for electrophysiology and imaging research. This fast perfusion system is designed to meet the needs of basic science and drug discovery researchers. Software control of all perfusion parameters makes the OctaFlow II ™ superior to all other perfusion systems.

Key features of the OctaFlow II ™ include:

    • Choice of low-maintenance pinch valves or fast-response solenoid valves
    • Valve-control of solution flow instead of motorized manipulation of barreled pipettes, minimizing sample losses following solution exchange
    • Keyboard solution selection, for exchange “on the fly”
    • Trigger in /out for synchronization with data acquisition systems
    • Preprogrammed solution delivery through software
    • Rapid flush mode for easy cleaning
    • Programmable pressurization of fluids for optimal solution flow
    • Programmable analog outputs for valve identification, step number, or pressure information to data acquisition

Each OctaFlow II ™ system allows for a choice of 8, 16, 24 or 32 reservoirs, which can be configured in different modes, depending on the experiment(s). Constant perfusion can be achieved through the combination of compound application and wash solution for minimization of solution convergence in manifold. Periodic application of compounds can be achieved through specific focal application. U-tube and J-tube configurations are also possible with the Octaflow II™.

The OctaFlow II ™ software runs on Windows 7 (32-bit) or Windows XP and communicates with the VC3 electronic valve controller via USB 2.0. Sequence steps are loaded at USB 2.0 speeds for fast editing and executing of sequence files. The graphical interface is designed to control all perfusion parameters from a single window. It also allows you to create configuration files and sequence files and to save them for future use. The Cleaning Manager function in the software allows for fast cleaning of reservoirs, valves and tubing.

Details

Details

Each OctaFlow II ™system includes the following:

  • Magnetic stand(s) with poles
  • Valve manifold(s) with solenoid or pinch valves and 5mL pressurized syringe reservoirs
  • Flush valve(s)
  • Quartz MicroManifold® perfusion outlet manifold(s) in various configurations (see below)
  • 230um ID and 600um OD Teflon tubing (FEP-1) and compression fittings (CF-1) for 1/16″ ID tubing (other sizes available – see below)
  • USB cable and software

Each OctaFlow II ™ system features a choice of valves:

  • Pinch valves : The 3-way normally open and normally closed pinch valves require minimal maintenance in long-term perfusion experiments and have response times of 15-20 ms.
  • Solenoid valves : The 2-way normally closed solenoid valves are optimized for high-speed solution exchange. These valves feature a biologically inert internally wettable surface and have response times of 1-2 ms.

Additional options include:

  • CF-2: Compression fitting for PE-20/FEP-2 tubing to 1/16″ ID tubing
  • FEP-2: Clear Teflon tubing with 380um ID and 760um OD
  • QMM-8: Quartz MicroManifold® 8 to 1 channel perfusion outlet manifold with 100um or 200um ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-16: Quartz MicroManifold® 16 to 1 channel perfusion outlet manifold with 100um or 200um ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-24: Quartz MicroManifold® 24 to 1 channel perfusion outlet manifold with 100um or 200um ID polyimide-coated quartz tubing and tip (other sizes available)
  • QMM-32: Quartz MicroManifold® 32 to 1 channel perfusion outlet manifold with 100um or 200um ID polyimide-coated quartz tubing and tip (other sizes available)

All of the Quartz MicroManifold® perfusion outlet manifolds are also available with an additional channel with 200um ID tube for flushing dead space and/or with Teflon tubing installed.

Ordering

Ordering

Part No. Description
ALA SPK-8 8 Channel Simple Perfusion Kit: magnetic stand with poles, reservoir bracket, drip chambers, 8 channel perfusion outlet manifold, manual 3-way luer valves, manual pinch flow valves, tubing and accessories
ALA 60MLRESCLP Set of four 60mL reservoir mounting clips with screws
ALA RESBRACK-8 8 Reservoir bracket for 60mL reservoirs with mounting block – other sizes available
ALA MMF-8 MiniManifold TM 8 to 1 channel perfusion outlet manifold made of 1/16″ ID ABS plastic
ALA 60mLSYRINGE Box of 25 60mL syringes
ALA SPK-DRIP Replacement drip chambers – pack of 10
ALA 3WLV Manual 3-way stop cock luer valve
ALA FCV-1 Manual pinch flow valve
ALA VM-STAND Replacement valve manifold stand: magnetic base and 2 rods
ALA SFK Miscellaneous tube and luer fittings
ALA TUBING-2 10′ 1/16″ ID x 1/8″ OD PVC tubing

 

Technical Data

Technical Data

Maximum number of perfusion valves 32
Suction developed via internal Venturi pump 199 mmHg nominal
Maximum number of valves per bank 8 valves/bank, up to 4 banks
Typical speed of pressure rise 520mmHg/sec
Maximum current output per valve 100mA/ 350mA solenoid/pinch valve
Pressures selectable in mm/Hg in increments of 1 or other units selectable
Maximum voltage/valve 18V
Sample flow rate 1mL in 9 min. @ 520 mmHg with standard QMM
Maximum on time per valve no limit
Minimum on time per valve 7ms @ 12V & 1ms @ 18V
Standard QMM 8 tubes with 100μm ID, 1 tube with 200μm ID, 100μm ID tip
Maximum input pressure 3970mmHg – other units selectable
Tip sizes available 100, 150, 200 μm ID
Maximum pressure applied ~800mmHg
Maximum number of sequence steps 300
Power requirements 120/220V
Maximum number of sequence events with looping 30020
OctaFlow interface 19″x3.5″x10″, 10 lbs/4.5kg
Infinite Step Function in Repeat Mode
Programmable Voltage Range 1-22volts/valve
USB 2.0 Support Software is compatible with Windows 7 and Windows XP