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TEC - Two Electrode Volgtag Clamp Amplifiers

TEC – Two Electrode Clamp – For Xenopus Oocytes and Neuromuscular Junction

TEC electrophysiology amplifiers are designed for two electrode clamp recordings in large cells like oocytes from the African claw frog Xenopus laevis. They can perform voltage clamp and current clamp and one special version (TEC-05X) even has a bridge mode.
npi’s TEC amplifiers have a high-voltage current source which allows fast voltage clamp and current clamp with high currents. The built-in PI controller guarantees quick and accurate responses to command steps.

Check out our tutorial videos about the TEC-03X on YouTube

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Your Benefits:

  • Easy setup: two headstages, no virtual ground is necessary.
  • Automatic “Oscillation Shutoff” prevents cells from damage.
  • Easy connection to all major data acquisition systems.
  • Fastest TEVC clamp speed through high voltage outputs.

Frequently asked questions (FAQ):

Why do I need two electrodes for Xenopus oocytes

Large cells have a large membrane surface and thus a low resistance over the whole membrane. The two-electrode voltage clamp technique (TEVC) separates the detection of the potential from the injection of currents by using distinct electrodes and headstages for each. This way both parameters can be recorded and controlled precisely.

What is the maximum current I can clamp with the TEC?

The exact maximum current depends on the resistance of the current electrode and is calculated by Ohm’s law: the current headstage of the TEC-03X/TEC-10CX has a maximum output of ±150 V. A typical current electrode has ~1 MΩ, the Oocyte in a high conducting state has a few kΩ. The maximum current in this case is ~150 µA.

How does the recording without “virtual ground” work?

The TEC two electrode amplifiers use a high-voltage current source in the current headstage and a differential amplifier in the potential headstage. This way the current is measured “en route” to the current electrode. This configuration eliminates artifacts induced by microelectrodes. The current injecting electrode is fully compensated and the introduction of a virtual ground is not necessary.

What other cells can I record with the TEC amplifiers?

The TEC-05X with its low voltage headstages is used for various types of large cells. This amplifier is used for neuromuscular junction recordings, muscle cells, vertebrate ganglion and other similar approaches.